Adenosine washing improves the retention rate of fat grafts under conditions of obesity

초록

Background Fat grafting is a commonly employed aesthetic procedure for contour en hancement. However, outcome prediction is challenging due to the complex regenera tion and remodeling processes involved. We investigated whether adenosine improves engraftment and fat graft survival under conditions of obesity. Methods Fat was harvested from mice fed a high-fat diet. This fat was washed with ei ther Krebs-Ringer bicarbonate HEPES buffer (the vehicle group) or a buffer containing 500 nM adenosine (the adenosine wash group). Subsequently, the fat was transplant ed into normal mice at 0.2 mL per mouse. In both groups, 50% of the mice were sacri ficed at 1 week and the remainder at 4 weeks post-transplantation. Reverse transcrip tion-quantitative polymerase chain reaction (RT-qPCR) analysis was conducted during week 1. In week 4, micro-computed tomography, immunofluorescence staining, and RT-qPCR were performed. A sample of the initially harvested fat was set aside for lipol ysis assay. Results Adenosine washing improved fat graft retention volumes by up to 50%. One week post-transplantation, the expression of adipogenic and angiogenic genes was found to be upregulated in the adenosine wash group. After 4 weeks, immunofluores cence staining revealed greater adipocyte integrity and an increased number of ves sels. Furthermore, adenosine appeared to modulate inflammation by stabilizing the li polysis rate. Conclusions Adenosine washing increased the fat graft survival rate under conditions of obesity. Clinically, this suggests a simple, cost-effective adjuvant method for improv ing fat graft survival in individuals with obesity. Further research is warranted to eluci date the underlying mechanisms and explore the applicability of this technique for au tologous transplantation.

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