Isolation and characterization of anti-inflammatory compounds from Sargassum horneri via high-performance centrifugal partition chromatography and high-performance liquid chromatography

초록

Sargassum horneri, a type of brown seaweed, has become popular in Korea over the past few years due to its massive influx from China. The present study is implemented towards developing methods for sustainable use of S. horneri biomass. Methanol extracts of S. horneri China (MSC) strain were investigated in order to isolate their bioactive natural products. Extracts were fractionated by solvent partitioning, high-performance centrifugal partition chromatography (HPCPC), and preparative high-performance liquid chromatography (prep-HPLC). Three norisoprenoids ((-)-loliolide, 3-hydroxy-5,6-epoxy-beta-ionone (HEBI), and apo-9'-fucoxanthinone) were isolated from chloroform fractions of MSC, and their structures were elucidated by nuclear magnetic resonance (NMR) spectroscopy. Solvent conditions for the HPCPC separation were optimized as hexane, ethyl acetate, methanol, and water in a ratio of 5:5:5:5. The isolated compounds displayed potential anti-inflammatory properties in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. For the first time, the three compounds reported herein were isolated from S. horneri. With further studies, S. horneri-derived extracts and natural products could be developed as cosmetics and/or functional food materials.

키워드