Toxicoproteomic identification of TiO2 nanoparticle-induced protein expression changes in mouse brain

초록

A proteomic analysis of the proteins in mouse brain that were differentially expressed in response to TiO2 nanoparticles was conducted to better understand the molecular mechanism of TiO2 nanoparticle-induced brain toxicity at the protein level. A total of 990 proteins from mouse brain were resolved by two-dimensional gel electrophoresis. A comparative proteomic analysis revealed that the expression levels of 11 proteins were changed by more than 2-fold in response to TiO2 nanoparticles: eight proteins were upregulated and three were downregulated by TiO2 nanoparticles. In addition, the activities of several antioxidative enzymes and acetylcholine esterase were reduced in TiO2 nanoparticle-exposed mouse brain. The protein profile alterations seem to be due to an indirect effect of TiO2 nanoparticles, because TiO2 nanoparticles were not detected in the brain in this investigation.

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