Transcriptomic Features and Odorant Receptor Discovery in the Endangered Predatory Beetle Damaster mirabilissimus mirabilissimus

초록

We generated a de novo transcriptome for the endangered predatory ground beetle Damaster mirabilissimus mirabilissimus using the Illumina HiSeq 4000 platform (150-bp paired-end). Sequencing produced 66,553,052 raw reads (approximate to 10 Gbp), of which 66,253,375 remained after quality filtering. Trinity assembly yielded an N50 of 2643 bp. Assembly completeness assessed with BUSCO (Insecta_odb9 dataset; 1367 orthologs) indicated 96.5% complete and 3.1% missing genes, supporting high overall quality. Functional annotation was performed against the PANM, Swiss-Prot, UniGene, and KOG databases, and functional classification employed KOG, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and InterProScan. We further identified 2050 transcriptome-derived simple sequence repeats (SSRs), with the dinucleotide motif AC being the most frequent (n = 77). Collectively, this dataset provides a primary functional-genomic resource for a nonmodel, threatened insect lacking a reference genome, delivers a candidate set for chemosensory gene families, including odorant receptors (ORs), and establishes a foundation for subsequent in vitro deorphanization and field-based chemical-ecology validation.

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