A novel fibrous scaffold composed of electrospun porous poly(epsilon-caprolactone) fibers for bone tissue engineering

초록

In this study, porous poly(-caprolactone) (PCL) fiber-based fibrous scaffolds are created using a suitable ratio of dimethyl chloride and acetone by using electrospinning. With the porous structure, it induced CaP particles to easily coat on the fibers after immersion in simulated body fluid solution. The morphology of the electrospun membranes was observed using scanning electron microscopic observation. The results showed that the CaP coated successfully, as examined by scanning electron microscopy, energy-dispersive spectroscopy, X-ray diffraction, transmission electron microscopic techniques and mass comparative analysis. The wettability of the coated fibrous scaffolds was tested using contact angle analysis. The invitro cellular proliferation and cell interaction with fibrous scaffolds were investigated. In addition, the invivo bone formation capacity of fibrous scaffolds including the non-porous (PCL-DCM), porous (PCL-DCM/Ace) and CaP coating on PCL/DCM-Ace for 2, 4, 8 and 12h immersed in SBF solution were also investigated. By measuring the invitro results, we verified that porous PCL fiber-based fibrous scaffold after 12h of immersion in simulated body fluid (PCL-DCM/Ace-12) was excellent for cell interaction, growth and proliferation. The invivo analyses showed that the PCL-DCM/Ace-12 fibrous scaffold enabled greater acceleration of bone formation than PCL/DCM and PCL/DCM-Ace fibrous scaffolds.

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